Chinese translation for "sephadex g"
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- 葡聚糖凝胶g
Related Translations:
sephadex: 降胆葡胺交联葡聚糖凝胶葡聚糖凝胶商品名葡奈糖展胶塞法戴克斯一种交联葡聚糖的商品名称
- Example Sentences:
| 1. | Mt - were prepared from the rabbit liver induced by zinc after isolation and purification . they were demetalled in acidic condition , then applied sephadex g - 25 to gain apomts free of metals
从锌诱导的家兔肝脏中分离纯化得到两种亚型的金属硫蛋白mts ： zn | | 2. | An active metabolite was obtained by purification with precipitated by ethanol , sephadex g - 25 gel , deae - cellulose ion exchange resin and silica gel column chromatography
经乙醇( 95 )沉淀、 sephadexg - 25凝胶层析、 deae -纤维素离子交换层析和硅胶层析纯化，得到抑制黑曲霉生长的单一组分。 | | 3. | In order to attain bioactive glycoprotein , glycoprotein of the leaves of camellia sinensis , was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography , by cona - sepharose 4b affinity chromatography
为了纯化天然糖蛋白，进行了茶树叶糖蛋白的cona - sepharose4b亲和层析，从sephadexg - 100凝胶过滤收集的粗糖蛋白中，分离茶树叶糖蛋白。 | | 4. | 3 . the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100 . the specific activity of the purified enzyme was increased by 37 . 9 folds over crude extract , and the recovery yield was 8 . 9 %
以粗酶液为起始，经硫酸铵分级沉淀、 sephadexg - 100凝胶过滤后，分离纯化了该酶，纯化倍数约为37 . 9 ，酶活力回收率8 . 9 。 | | 5. | The crude cellulases from liquid fermentation of b - 6 and ass . 3711 were isolated and purified by ( nh4 ) so4 precipitation , sephadex g - 100 and deae - sepharose cl 6b column chromatography . the cmcase components were purified and some of their physical and chemical properties were studied
本文将液体发酵的酶液经硫酸铵分级沉淀、柱层析后得电泳纯cmcase组分，并对as3 . 3711和b - 6来源的cmcase酶解动力学和理化性质作了比较研究。 | | 6. | An antifungal protein , named as b16 , was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate , desalt and gel filtration on sephadex g - 75 , which with molecular weight at about 30 - 40 kd on the basis of sds - page
菌株041381发酵上清液经70饱和度硫酸铵沉淀，透析脱盐， sephadexg - 75凝胶过滤层柱，得到活性物质b16 。以sds - page胶为基础进行电泳分析， b16的分子量为30 - 40kd 。 | | 7. | The antimicrobial secretion could be divided into three sections , every section had antimicrobial activity . the first could be extracted by petroleum ether , the second could be extracted by ethyl alcohol , the third could dissove in water and could be separated by sephadex g - 50 , g200 gel filtration chromatography and carbornmethyl cellulose - 1 anion - exchange chromatography and detected by a256 , . the antimicrobial secretion had wide spectrum and had strong inhitory activity against germs and fungi , they could inhibit sixteen kinds of plant pathogenic germs , eight kinds of animal germs and eight kinds of plant pathogenic fungi
粗提物经石油醚萃取可得第一个活性部分，剩余部分经无水乙醇萃取可得第二个活性部分，剩余物质再经凝胶sephadexg - 50后有两个峰，第二峰有活性，再经凝胶sephadexg - 200后有三峰，第二峰有活性，将其经过阳离子交换柱cm - 1后有两峰，此两峰均有抑菌活性。 | | 8. | After the acet is vaporized , the active substance in water is gotten . and which is vaporized at low temperature . then the crude active substance is purified by column chromatography on sephadex g - 75 . after a series of purifications again , we could get some white powder at last . though the active substance is diluted to50 g / ml , the activity is still checkeded - up through phyto phtnora casicileon . the purified active substance is insensitive to heat , resistant to chloroform 、 ethanol and the orhers . in addition , the active substance is sensitive to high ph ( 10 ~ 14 ) , but it is not sensitive to low ph ( 1 ~ 5 ) . furthermre , when the ph is made to low again , the activity of it ' s comes back
用蒸馏水对菌体稀释；加入适量吸附树脂在150rpm 、 28下振荡吸附4h ， 80 %的丙酮解吸，过滤解吸液得到活性物质的澄清溶液，旋转蒸发仪旋转蒸发去处丙酮，经sephadexg - 75分子筛层析得单一活性峰，收集峰值部分样品液经冷冻干燥得到淡褐色粉末，该活性物质用丙酮充分洗涤、甲醇-乙醚重结晶获得略带微黄的白色粉末，该活性物质50 g / ml仍可对苏云金芽孢杆菌hd - 1产生明显的抑制作用。 | | 9. | Then enzyme was purified with a deae - cellulose ( 5 . 5x50cm ) column , a toyopearl hw - 65 ( 5 . 5 x 50cm ) column and a sephadex g - 200 ( 5 . 5 x 80cm ) column . finally , the enzyme was purified for 10 folds with the recovery of 17 . 4 % . page showed a single band for the purified creatinase
3 、肌酸水解酶的提纯酶在硫酸铵饱和度为40 80之间完全沉淀，先后经过deae - cellulose离子层析柱、 toyopearlhw - 65疏水层析柱、 sephadexg - 200分子筛层析柱层析，最终使酶提纯10倍，最终得率为17 . 4 。 | | 10. | 6 - phosphogluconate dehydrogenase ( 6 - pgadase , ec 1 . 1 . 1 . 44 ) was isolated by homogenate , ammunium sulfate fractionation , deae - sepharose chromatography , blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis , and some properties of the enzyme had been studied . a 113 . 8 - fold purification was obtained with a 8 . 2 % yield . the purified enzyme moved as a single electrophoretic band in page
将枯草芽孢杆菌超声波破壁后的粗提取物进行分段盐析、 deae - sepharose阴离子交换柱层析， blue - sepharosecl - 6b特异结合柱层析和sephadexg - 200凝胶过滤等纯化步骤，得到聚丙烯酰胺凝胶电泳为单一蛋白区带，比活为1 . 46u mg的酶制剂。 |
- Similar Words：
- "sephacel" Chinese translation, "sephadex" Chinese translation, "sephadex chromatography" Chinese translation, "sephadex column" Chinese translation, "sephadex column chromatography" Chinese translation, "sephadex g-100" Chinese translation, "sephadex gel" Chinese translation, "sephadex ion exchanger" Chinese translation, "sephadex microsphere" Chinese translation, "sephard" Chinese translation
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